The Addition of Biochar and Hyper-Thermal Inoculum Can Regulate the Fate of Heavy Metals Resistant Bacterial Communities during the Livestock Manure Composting

In the present investigation the effects of biochar and hyper-thermal inoculum on the heavy-metal-resistant bacteria (HMRB) during livestock manure composting were studied. An experiment was performed on composting livestock manure and wheat straw amended with biochar and hyper-thermal inoculum. Physicochemical properties, enzyme activity, heavy metals (HMs), and bacterial activities were monitored, and a comprehensive assessment was analyzed during the composting process. The results showed that the dominant phyla of Proteobacteria, Bacteroidota, Actinobacteriota, and Chloroflexi were enriched, but this was not the case with Firmicutes. The abundance of Galbibacter, Thermobifida, Sphaerobacter, and Actinomadura was significantly different in CT15 and BHCT15. In addition, this study showed that the selected factors are less correlated with HMRB compared with the CT group. Therefore, this study could provide new insights into the effect of biochar and hyper-thermal inoculum amendments on the fate of HMRB under HMs and high temperature stress during livestock manure composting.