Nocodazole induces mitotic cell death with apoptotic-like features in Saccharomyces cerevisiae

被引:21
|
作者
Endo, Kingo [1 ]
Mizuguchi, Mayumi [1 ]
Harata, Aoi [1 ]
Itoh, Go [1 ]
Tanaka, Kozo [1 ]
机构
[1] Tohoku Univ, Inst Dev Aging & Canc, Aoba Ku, Sendai, Miyagi 9808575, Japan
基金
日本科学技术振兴机构;
关键词
Budding yeast; Nocodazole; Mitotic cell death; Apoptosis; Mitotic arrest; SPINDLE-ASSEMBLY CHECKPOINT; ANTIMITOTIC DRUGS; YEAST APOPTOSIS; CANCER-CELLS; MICROTUBULES; CASPASES; DIVISION; ARREST; TARGET;
D O I
10.1016/j.febslet.2010.04.029
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We investigated the fate of budding yeast treated with nocodazole, a microtubule-depolymerizing drug. Cells died after mitotic arrest while staying in mitosis, suggesting that mitotic cell death, but not mitotic slippage, mainly occurs in nocodazole-treated cells. Nocodazole-treated cells showed features of apoptotic-like cell death, but not those of cell lysis or autophagy. Consistently, mitochondria-dependent production of reactive oxygen species was involved in the cell death. Similar cell death was also seen in cells after mitotic arrest by perturbation of the anaphase-promoting complex/cyclosome. In addition, caspase activity was found in nocodazole-treated cells, which was independent of the metacaspase, Mca1. Our results suggest that budding yeast can be a model to study mitotic cell death in cancer treatment with antimitotic drugs. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
引用
收藏
页码:2387 / 2392
页数:6
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