Isolation and cellular fatty acid profile analyzation of two marine bioluminescent bacteria

Two luminescent bacterial strains KOOS1 land KOOS2 isolated from surface mucus of Octopus sp. collected from Andaman were identified by their cellular fatty acid composition analyzation with the help of Microbial Identification system (MIDI). SIM indexes obtained for these isolated strains were 0.772 (KOOS1) and 0.754 (KOOS2) respectively and were identified as Photobacterium damselae and Vibrio fischeri. Major fatty acids found in Photobacterium damselae were Saturated: Dodecanoic acid (C-12:0), Tetradecanoic acid (C-14.0), Pentadecanoic acid (C-15:0), Hexadecanoic acid (C-16:0), Heptadecanoic acid (C-17:0) and Octadecanoic acid (C-18:0); and Unsaturated: 3-hydroxy-9-methyl decanoic acid (C(11:0)iso 3OH), 3-hydroxydodecanoic(C-12:0 3OH), C-16:1 omega 5c, Oleic acid (C-18:1 omega 9c) and C-18:1 omega 5c.In Vibrio fischeri Saturated: C-12.0, Tridecanoic acid (C-13.0), C-15:0, C-16:0, C-17:0 and C-18:0; and Unsaturated: C(11:0)iso 3OH,2-hydroxydodecanoic (C-12.0 2OH), C(12:0)3OH, C(13:0)iso, C(14:0)iso, C(15:0)anteiso, C(16:0)iso, Cnoiso, C(17:0)iso, C-16:0 omega 5c,C-15:0 iso3OH, C-17:1 omega 8c and C-17:1 omega 6c were found. Cyclopropane acids have not been detected in both Photobacterium damselae and Vibrio fischeri.