The variation rule and mechanism of microstructure and texture of 6016 aluminum alloy during cold rolling was studied using the electron back-scatter diffraction technology (EBSD) combined with SEM+EDS and XRD. The results show that with the increase of cold rolling passes, grains constantly elongate along the rolling direction, and inhomogeneous deformation phenomenon between different grains occurs first but gradually disappears later, and catenation of second phase particles are crushed by metal plastic flow; with the deformation increasing, small angle dislocation interfaces increase, and they have an angle about 40 to the rolling direction from low to medium strains, but some of them are induced to large angle interfaces by large strains, which cause the deformation grains fragmentation; at the initial rolling stage, overall coordinate deformation and rotation of grains form some deformation texture, and in further deformation, areas near the grain boundary and interior of grains are easy to rotate from grain's initial orientation to the beta orientation line. It is the two mechanisms that increase the deformation textures, led by S texture, and decrease the Cube texture constantly.