Development and Characterization of Wheat-rye Lines Combining T1RS.1BL Translocation and 5R(5D) Chromosome Substitution or T1RS.1BL and T5AS.5RL Translocations

As a result of crossing substituted lines Saratovskaya 29 (S29) 5R(5A) and S29 5R(5D) with line L2075 (T1RS.1BL), two homozygous wheat-rye lines were obtained in the F-7 generation and identified as T5AS.5RL + T1RS.1BL and 5R(5D) + T1RS.1BL, respectively. The rye chromosomes yare of different origins: 5R originated from spring rye Onohoskaya and 1RS from winter rye Saratovskaya 5. A new Robertsonian translocation was obtained where the 5RL arm was translocated to the short arm of wheat chromosome 5A, resulting in the T5AS.5RL translocation chromosome. Two translocations, T5AS.5RL and T1RS.1BL, and one chromosome substitution, 5R(5D), were identified and confirmed to be compensating on the basis of genomic in situ hybridisation, C-banding and 1RS-and 5R-specific PCR markers. Evaluation of resistance to fungal diseases revealed that homozygous T5AS.5RL + T1RS.1BL and 5R(5D) + T1RS.1BL lines are resistant to leaf rust and powdery mildew.