Molecular cloning of bovine Thyrotropin-Releasing Hormone Receptor gene

被引：12
|
作者：
Takata, M
Shimada, Y
Ikeda, A
Sekikawa, K
机构：
[1] Natl Inst Anim Hlth, Ibaraki, Osaka 305, Japan
[2] Miyazaki Med Coll, Dept Legal Med, Kiyotake, Miyazaki 88916, Japan
[3] Inst Soc Technoinnovat Agr Forestry & Fisheries, Ibaraki, Osaka 305, Japan
来源：
JOURNAL OF VETERINARY MEDICAL SCIENCE | 1998年 / 60卷 / 01期
关键词：
bovine; molecular cloning; Thyrotropin-Releasing Hormone Receptor;
D O I：
10.1292/jvms.60.123
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The genomic DNA encoding bovine Thyrotropin-Releasing Hormone Receptor (TRHR) was isolated from a bovine (Holstein) genomic library. Using PCR fragments of bovine candidate TRHR Transmembrane domain (TMD-III-IV) and C-terminus domain of mouse TRHR cDNA as probes, 9 x 10(5) plaques were screened to obtain several clones each containing the N-terminus or C-terminus domain. The bovine TRHR gene encoded 398 amino acids and has a long intron. The identity of the deduced amino acid sequence of bovine TRHR exceeded 88% that of mouse, rat or human. RT-PCR analysis indicated TRHR mRNA to be expressed in the pituitary and brain.
引用
收藏
页码：123 / 127
页数：5
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