Quantitative sample injection for capillary electrophoresis

被引：20
|
作者：
Yao, CY
Gao, RY
Yan, C
机构：
[1] Unimicro Technol Inc, Pleasanton, CA 94566 USA
[2] Nankai Univ, Inst Elementoorgan Chem, State Key Lab, Tianjin 300071, Peoples R China
[3] Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116011, Peoples R China
来源：
JOURNAL OF SEPARATION SCIENCE | 2003年 / 26卷 / 1-2期
关键词：
quantitative sample injection; capillary electrophoresis; capillary electrochromatography; pressurized CEC; micro-HPLC;
D O I：
10.1002/jssc.200390011
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An apparatus including a rotary-type injector was designed for quantitative sample injection in capillary electrophoresis (CE), in which both pressurized flow and electroosmotic flow were used to drive the background electrolyte solution. A relative standard deviation of peak area of lower than 1% was achieved by using this apparatus. The effects of back-pressure regulator, restrictor, and applied voltage on separation efficiency and resolution were investigated. The utility of this apparatus in both micro-HPLC and pressurized capillary electrochromatography (pCEC) was also demonstrated.
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页码：37 / 42
页数：6
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