Parachryseolinea silvisoli gen. nov., sp. nov., isolated from forest soil, and reclassification of Chryseolinea flava as Pseudochryseolinea flava gen. nov., comb. nov.

A lemon-chiffon strain, designated QH1ED- 6- 2(T), was isolated from a soil sample collected from Qinghai Virgin Forests, Qinghai Province, PR China. The strain was Gram- stain-negative, aerobic, rod-shaped and motile by gliding. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain QH1ED- 6- 2T belongs to the family Fulvivirgaceae, and has the highest similarity values of 93.6-92.0 % to Ohtaekwangia koreensis CCUG 58939(T), Ohtaekwangia kribbensis CCUG 58938(T), Chryseolinea flava SDU1-6(T) and Chryseolinea serpens DSM 24574(T), respectively. The major cellular fatty acids included iso-C15:0, C16:1 ?5c, iso-C17: 0 3 -OH and summed feature 3. The major polar lipid was phosphatidylethanolamine. The predominant respiratory quinone was menaquinone- 7. The average amino acid identity values and percentages of conserved proteins between QH1ED- 6- 2(T) and its closely related genera were 66.4-69.6 % and 58.9-64.9 %, respectively, which are interspersed in the intra-genera cutoff values. The digital DNA-DNA hybridization values were 17.6-19.2 %. The draft genome size of strain QH1ED- 6- 2(T) was 7.98 Mbp with a DNA G+C content of 51.4 mol%. Based on phenotypic, chemotaxonomic, phylogenetic data, genomic DNA G+C content, as well as AAI, POCP and dDDH results, strain QH1ED- 6- 2(T) represents a novel species of a new genus in the family Fulvivirgaceae, for which the name Parachryseolinea silvisoli sp. nov. is proposed. The type strain is QH1ED- 6- 2(T) (=GDMCC 1.2318(T)=JCM 35041(T)). We also propose the reclassification of Chryseolinea flava as Pseudochryseolinea flava gen. nov., comb. nov. (type strain SDU1-6(T)=CGMCC 1.13492(T)=JCM 32520(T)).