In-Capillary Chiral Derivatization of Amino Acids

被引：5
|
作者：
Kartsova, L. A. [1 ]
Moskvichev, D. O. [1 ]
机构：
[1] St Petersburg State Univ, Inst Chem, St Petersburg 198504, Russia
来源：
JOURNAL OF ANALYTICAL CHEMISTRY | 2022年 / 77卷 / 05期
基金：
俄罗斯科学基金会;
关键词：
amino acids; capillary electrophoresis; chiral derivatization; culture liquid; preconcentration; CHROMATOGRAPHIC ENANTIOSEPARATION; ORTHO-PHTHALALDEHYDE; ELECTROPHORESIS;
D O I：
10.1134/S1061934822050057
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The capabilities of in-capillary chiral derivatization of amino acids using ortho-phthalaldehyde and N-acetyl-L-cysteine are discussed. The influence of the concentration of a borate buffer solution and the addition of 2-hydroxypropyl-beta-cyclodextrin and alpha-cyclodextrin to the supporting electrolyte on the selectivity of separation of the resulting diastereomeric derivatives was revealed. The found conditions were adapted to the electrophoretic analysis of the culture liquid of Escherichia coli bacteria for the detection of amino acids produced by them. Tyrosine, alanine, serine, and glutamic acid were identified. An important result is the detection of D-serine and D-alanine in the culture liquid.
引用
收藏
页码：618 / 624
页数：7
相关论文
共 50 条
  • [1] In-Capillary Chiral Derivatization of Amino Acids
    L. A. Kartsova
    D. O. Moskvichev
    Journal of Analytical Chemistry, 2022, 77 : 618 - 624
  • [2] Development of an in-capillary derivatization method by CE for the determination of chiral amino acids in dietary supplements and wines
    Belen Martinez-Giron, Ana
    Garcia-Ruiz, Carmen
    Crego, Antonio L.
    Luisa Marina, Maria
    ELECTROPHORESIS, 2009, 30 (04) : 696 - 704
  • [3] Determination of amino acids by high-performance capillary electrophoresis with on-line mode in-capillary derivatization
    Oguri, S
    Yokoi, K
    Motohase, Y
    JOURNAL OF CHROMATOGRAPHY A, 1997, 787 (1-2) : 253 - 260
  • [4] Chemo- and enantio-selective method for the analysis of amino acids by capillary electrophoresis with in-capillary derivatization
    Fradi, Ines
    Servais, Anne-Catherine
    Lamalle, Caroline
    Kallel, Mohamed
    Abidi, Mbarek
    Crommen, Jacques
    Fillet, Marianne
    JOURNAL OF CHROMATOGRAPHY A, 2012, 1267 : 121 - 126
  • [5] Sensitivity enhancement by on-line preconcentration and in-capillary derivatization for the electrophoretic determination of amino acids
    Latorre, RM
    Saurina, J
    Hernández-Cassou, S
    ELECTROPHORESIS, 2001, 22 (20) : 4355 - 4361
  • [6] In-capillary derivatization with (-)-1-(9-fluorenyl)ethyl chloroformate as chiral labeling agent for the electrophoretic separation of amino acids
    Fradi, Ines
    Farcas, Elena
    Ben Said, Azza
    Yans, Marie-Laure
    Lamalle, Caroline
    Somsen, Govert W.
    Prior, Amir
    de Jong, Gerhardus J.
    Kallel, Mohamed
    Crommen, Jacques
    Servais, Anne-Catherine
    Fillet, Marianne
    JOURNAL OF CHROMATOGRAPHY A, 2014, 1363 : 338 - 347
  • [7] A micellar electrokinetic chromatography-mass spectrometry approach using in-capillary diastereomeric derivatization for fully automatized chiral analysis of amino acids
    Moldovan, Radu-Cristian
    Bodoki, Ede
    Kacso, Timea
    Servais, Anne-Catherine
    Crommen, Jacques
    Oprean, Radu
    Fillet, Marianne
    JOURNAL OF CHROMATOGRAPHY A, 2016, 1467 : 400 - 408
  • [8] Strategies for in-capillary derivatization of amino acids in capillary electrophoresis using 1,2-naphthoquinone-4-sulfonate as a labeling reagent
    Latorre, RM
    Hernández-Cassou, S
    Saurina, J
    JOURNAL OF CHROMATOGRAPHY A, 2001, 934 (1-2) : 105 - 112
  • [9] In-capillary derivatization and analysis of amino acids, amino phosphonic acid-herbicides and biogenic amines by capillary electrophoresis with laser-induced fluorescence detection
    Molina, M
    Silva, M
    ELECTROPHORESIS, 2002, 23 (14) : 2333 - 2340
  • [10] In-capillary derivatization and capillary electrophoresis separation of amino acid neurotransmitters from brain microdialysis samples
    Denoroy, Luc
    Parrot, Sandrine
    Renaud, Louis
    Renaud, Bernard
    Zimmer, Luc
    JOURNAL OF CHROMATOGRAPHY A, 2008, 1205 (1-2) : 144 - 149
12345