Determination of 33 Pesticide Residues in Honeysuckle by Gel Permeation Chromatography Purification and Gas Chromatography-Mass Spectrometry

A method was developed for the simultaneous determination of 33 pesticide residues, including organochlorine, organophosphorus and pyrethroid pesticide, in honeysuckle by gel permeation chromatography (GPC) purification and gas chromatography-mass spectrometry (GC-MS) detection. The pesticides were extracted using mixed solvent of ethyl-acetate and cyclohexane (1: 1, V/V). The pesticides extracted from sample were separated and purified with matrices such as pigment by gel permeation chromatography. The pesticides were finally separated and detected by GC-MS with full scan and selective ion monitor mode simultaneously. The precisions of developed method with relative standard deviation for 33 pesticide were lower than 16. 7% (6. 9 - 84. 6 ng/g, n = 5). The recoveries for 33 pesticide standards spiked samples were between 57. 4% (methamidophos) and 109. 9% (malathion). Limits of detection (3 sigma) and quantifications (10 sigma) of the method were 0. 10 - 10.5 ng/g and 0.33 - 20.0 ng/g, respectively.