Structural Characterization and Comparison of Switchgrass Ball-milled Lignin Before and After Dilute Acid Pretreatment

被引：224
|
作者：
Samuel, Reichel [1 ,2 ]
Pu, Yunqiao [1 ,3 ]
Raman, Babu [1 ,4 ]
Ragauskas, Arthur J. [1 ,2 ,3 ]
机构：
[1] Oak Ridge Natl Lab, BioEnergy Sci Ctr, Oak Ridge, TN 37831 USA
[2] Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 USA
[3] Georgia Inst Technol, Inst Paper Sci & Technol, Atlanta, GA 30332 USA
[4] Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN USA
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2010年 / 162卷 / 01期
关键词：
Pretreatment; Switchgrass; Ball-milled lignin; HSQC; C-13 and P-31 NMR spectroscopy; NUCLEAR-MAGNETIC-RESONANCE; ENZYMATIC SACCHARIFICATION; WOOD LIGNINS; RICE HULLS; ENERGY; DELIGNIFICATION; FERMENTATION; HYDROLYSIS; EXTRACTION; CONVERSION;
D O I：
10.1007/s12010-009-8749-y
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
To reduce the recalcitrance and enhance enzymatic activity, dilute H2SO4 pretreatment was carried out on Alamo switchgrass (Panicum virgatum). Ball-milled lignin was isolated from switchgrass before and after pretreatment. Its structure was characterized by C-13, HSQC, and P-31 NMR spectroscopy. It was confirmed that ball-milled switchgrass lignin is of HGS type with a considerable amount of p-coumarate and felurate esters of lignin. The major ball-milled lignin interunit was the beta-O-4 linkage, and a minor amount of phenylcoumarin, resinol, and spirodienone units were also present. As a result of the acid pretreatment, there was 36% decrease of beta-O-4 linkage observed. In addition to these changes, the S/G ratio decreases from 0.80 to 0.53.
引用
收藏
页码：62 / 74
页数：13
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