Multiplex genotyping of PCR products with MassTag-labeled primers

被引：74
|
作者：
Haff, LA
Smirnov, IP
机构：
[1] PerSeptive Biosystems, Inc., Framingham, MA 01701
来源：
NUCLEIC ACIDS RESEARCH | 1997年 / 25卷 / 18期
关键词：
D O I：
10.1093/nar/25.18.3749
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple mass spectrometric based assay, the pinpoint assay, has previously been described for typing single nucleotide polymorphisms. The identity of a polymorphism is determined by mass differences of single base extended genotyping primers as determined by MALDI-TOF mass spectrometry. A simple method for multiplexing the assay is described, employing multiple primers with 5' oligo(dT) sequences (MassTags) which serve to mass discriminate the peaks of multiple extended and non-extended primers. The assay is extremely rapid and requires no labeling reagents.
引用
收藏
页码：3749 / 3750
页数：2
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