EFFECT OF PLANT GROWTH REGULATORS ON IN VITRO PLANT REGENERATION OF WHEAT (Triticum aestivum L.) FROM EMBRYO EXPLANTS

被引:0
|
作者
Xhulaj, Doriana [1 ]
机构
[1] Agr Univ Tirana, Inst Plant Genet Resources, Tirana, Albania
来源
JOURNAL OF ANIMAL AND PLANT SCIENCES | 2019年 / 29卷 / 06期
关键词
embryo; direct shoot regeneration; growth regulators; rooting; TISSUE-CULTURE RESPONSES; SOMATIC EMBRYOGENESIS; CULTIVARS; SYSTEM; INDUCTION; CALLUS;
D O I
暂无
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
An efficient method was developed for multiple shoot regeneration of Albanian wheat (Triticum aestivum L.) cultivar "Dajti" from mature embryos without callus formation, for a short period of time. The effect of explants, isolated shoot embryonic meristem and shoot embryonic meristem with scutellum and various combinations of plant growth regulators in MS media on direct shoot regeneration of wheat was investigated. Among different combination of 2,4-D, IBA, NAA and BAP tested, embryo explants cultured in MS medium supplemented with 2 mg/L BAP, 0.6 mg/L 2,4-D resulted as the most efficient direct shoot regeneration and produces a maximum of 2.5 shoot per explants and 3.4 cm shoot length. Plantlets were successfully transferred to rooting medium. The greatest mean numbers of roots were obtained on MS media supplemented with 0.5 mg/L NAA, and the best mean value of root length (8.2 cm) at 0.5 mg/L IBA. In this paper by simple manipulating the concentrations of BAP, 2,4 D, IBA and NAA in the culture medium, is described a method for rapidly obtaining whole plants of wheat without the subculture, using embryo explants.
引用
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页码:1616 / 1621
页数:6
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