Transient expression of Arabidopsis thaliana ascorbate peroxidase 3 in Nicotiana benthamiana plants infected with recombinant potato virus X

被引：19

作者：

Escobar, C

Hernández, LE

Jiménez, A

Creissen, G

Ruiz, MT

Mullineaux, PM

机构：

[1] Univ Castilla La Mancha, Dept Biol Vegetal, Fac Ciencias Ambientales, Toledo 45003, Spain

[2] John Innes Ctr Plant Sci Res, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England

来源：

PLANT CELL REPORTS | 2003年 / 21卷 / 07期

关键词：

potato virus X; ascorbate peroxidase; over-expression;

D O I：

10.1007/s00299-002-0570-9

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We have explored the transient over-expression of Arabidopsis thaliana ascorbate peroxidase 3 (APX3) in Nicotiana benthamiana using a viral vector based on the potato virus X (PVX). Plants infected with a PVX:APX3 hybrid had a similar progression of viral particles compared to control plants infected with a PVX:GFP hybrid, indicating that infection was not affected by the over-expression of heterologous APX3. Our results also showed that in PVX:APX3-infected plants, the hybrid virus directed a high level of APX3 expression and the recombinant protein was functional, as inferred from the higher APX activity compared to mock and PVX:GFP hybrid-infected plants. The PVX recombinant expression system used is a simple and quick method for transient expression of heterologous APXs, which are expected to suffer specific processing in plant cells.

引用

页码：699 / 704

页数：6

共 46 条

[1] Transient expression of Arabidopsis thaliana ascorbate peroxidase 3 in Nicotiana benthamiana plants infected with recombinant potato virus X
C. Escobar
L. E. Hernández
A. Jiménez
G. Creissen
M. T. Ruiz
P. M. Mullineaux
Plant Cell Reports, 2003, 21 : 699 - 704
[2] Long-Term Potato Virus X (PVX)-Based Transient Expression of Recombinant GFP Protein in Nicotiana benthamiana Culture In Vitro
Sindarovska, Yana
Kuchuk, Mykola
PLANTS-BASEL, 2021, 10 (10):
[3] Plant virus expression systems for transient production of recombinant allergens in Nicotiana benthamiana
Wagner, B
Fuchs, H
Adhami, F
Ma, Y
Scheiner, O
Breiteneder, H
METHODS, 2004, 32 (03) : 227 - 234
[4] Identification of viral particles in the apoplast of Nicotiana benthamiana leaves infected by potato virus X
Hu, Shuzhen
Yin, Yueyan
Chen, Buyang
Lin, Qi
Tian, Yanzhen
Song, Xijiao
Peng, Jiejun
Zheng, Hongying
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MOLECULAR PLANT PATHOLOGY, 2021, 22 (04) : 456 - 464
[5] Heterologous production of the major apple allergen Mal d 1 in Nicotiana benthamiana plants transfected by a recombinant potato virus X based expression vector
Hoffmann-Sommergruber, K
Ma, Y
Krebitz, M
Zuidmeer, L
Van Ree, R
Scheiner, O
Breiteneder, H
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 2002, 109 (01) : S306 - S306
[6] Efficient transient expression of human GM-CSF protein in Nicotiana benthamiana using potato virus X vector
Fengyong Zhou
Ming-Li Wang
Henrik H. Albert
Paul H. Moore
Yun J. Zhu
Applied Microbiology and Biotechnology, 2006, 72 : 756 - 762
[7] Efficient transient expression of human GM-CSF protein in Nicotiana benthamiana using potato virus X vector
Zhou, Fengyong
Wang, Ming-Li
Albert, Henrik H.
Moore, Paul H.
Zhu, Yun J.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2006, 72 (04) : 756 - 762
[8] Transient expression of recombinant proteins in plants using potato virus X based vectors
Mardanova, Eugenia S.
Ravin, Nikolai V.
RECOMBINANT PROTEIN EXPRESSION: EUKARYOTIC HOSTS, 2021, 660 : 205 - 222
[9] Ribosome profiles and riboproteomes of healthy and Potato virus A- and Agrobacterium-infected Nicotiana benthamiana plants
Eskelin, Katri
Varjosalo, Markku
Ravantti, Janne
Makinen, Kristiina
MOLECULAR PLANT PATHOLOGY, 2019, 20 (03) : 392 - 409
[10] Production of Bioactive Recombinant Reteplase by Virus-Based Transient Expression System in Nicotiana benthamiana
Ma, Ting
Li, Zhiying
Wang, Sheng
FRONTIERS IN PLANT SCIENCE, 2019, 10

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