Decolorization Reaction of Dye Using Peroxidase in Eco-Friendly Washing

In recent years, eco-friendly washing was spread and neutralization of the washing bath and reduction of bath ratio is in progress. Since there is a possibility that dye transfer occurs during washing, decolorization reaction of dye catalyzed by peroxidase was examined to correspond to eco-friendly washing. Decolorization conditions that dye transfer of Acid Blue 113 and Direct Blue 71 using 3.4 x 10(-8) M horseradish peroxidase (HRP) doesn't occur were pH 7.0 Carmody buffer, over 2.5 x 10(-5) hydrogen peroxide and over 1.0 x 10(-5) M p-iodophenol. Decolorization reaction of both dyes with sodium percarbonate is conventional bleaching agent didn't proceed substantially. So, practical effectiveness of HRP as a detergent added enzyme was confirmed. When the surfactant concentration was cmc x 1.5, decolorization rate constant was reduced from 10% to 40%.