During the incineration of turkey manure, a wide variety and concentration of nutrients are conserved in the turkey manure ash (TMA). In particular, the high concentration of citrate-soluble phosphorus (P) (43 g kg-1) may make it a suitable P source for crop production. The ash is alkaline with a pH of 12.2. We conducted a soil incubation study using a low-P soil with a pH of 6.2 to evaluate the effects of TMA on soil pH and extractable P. Two TMA rates, based on citrate-soluble P (10.9 and 21.9 mg kg-1), were compared with equivalent rates of triple superphosphate (TSP). In addition, a 0-P control was included. At the rates tested, TMA slightly increased soil pH, but this increase would be of minor agronomic importance. At equivalent P rates, changes in water-soluble P (WSP) concentrations with TMA and TSP were similar. Changes in iron-oxide-strip-extractable P (FeO P) and Bray 1 P concentrations were greater with TMA than with TSP. In contrast, changes in Olsen P concentrations were greater with TSP than with TMA for up to 32 days. For TMA, the ability of the tests to extract P was ranked, from highest to lowest, as Bray 1 P FeO P Olsen P WSP, and for TSP they were ranked Bray 1 P epsilon Olsen P epsilon FeO P WSP. However, the Bray 1 P and FeO P tests tended to overestimate plant-available P because they measured more citrate-soluble P than that added with TMA. We conclude that TMA can be used as a P source for crop production and that the Olsen test may provide a better estimate of plant-available P in TMA-amended soil.