Quantitative Assessment of Sialo-Glycoproteins and NGlycans during Cardiomyogenic Differentiation of Human Induced Pluripotent Stem Cells

被引：22
|
作者：
Konze, Sarah A. [1 ,2 ]
Cajic, Samanta [3 ]
Oberbeck, Astrid [1 ,2 ]
Hennig, Rene [3 ,4 ]
Pich, Andreas [5 ]
Rapp, Erdmann [3 ,4 ]
Buettner, Falk F. R. [1 ,2 ]
机构：
[1] Hannover Med Sch, Inst Clin Biochem, Carl Neuberg Str 1, D-30625 Hannover, Germany
[2] Hannover Med Sch, REBIRTH Cluster Excellence, Carl Neuberg Str 1, D-30625 Hannover, Germany
[3] Max Planck Inst Dynam Complex Tech Syst, Sandtorstr 1, D-39106 Magdeburg, Germany
[4] glyXera GmbH, Leipziger Str 44, D-39120 Magdeburg, Germany
[5] Hannover Med Sch, Inst Toxicol, Carl Neuberg Str 1, D-30625 Hannover, Germany
来源：
CHEMBIOCHEM | 2017年 / 18卷 / 13期
关键词：
cardiomyocytes; glycoproteins; human pluripotent stem cells; proteomics; sialic acids; N-GLYCANS; SURFACE-MARKER; IDENTIFICATION; GLYCOSYLATION; GENE; GLYCOME; HEART; GLYCOSYLTRANSFERASE; QUANTIFICATION; CARDIOMYOCYTES;
D O I：
10.1002/cbic.201700100
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Human induced pluripotent stem-cell-derived cardiomyocytes (hiPSC CMs) may be used in regenerative medicine for individualized tissue transplants in the future. For application in patients, the generated CMs have to be highly pure and well characterized. In order to overcome the prevalent scarcity of CM-specific markers, we quantitatively assessed cell-surface-exposed sialo-glycoproteins and N-glycans of hiPSCs, CM progenitors, and CMs. Applying a combination of metabolic labeling and specific sialo-glycoprotein capture, we could highly enrich and quantify membrane proteins during cardiomyogenic differentiation. Among them we identified a number of novel, putative biomarkers for hiPSC CMs. Analysis of the N-glycome by capillary gel electrophoresis revealed three novel structures comprising beta 1,3-linked galactose, alpha 2,6-linked sialic acid and complex fucosylation; these were highly specific for hiPSCs. Bisecting GlcNAc structures strongly increased during differentiation, and we propose that they are characteristic of early, immature CMs.
引用
收藏
页码：1317 / 1331
页数：15
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