Online Determination of Oxygen Uptake and Carbon Dioxide Production Rates in Mammalian Cell Culture Using Mass Spectrometry

被引：1

作者：

Haas, J. ^{[1
]}

Trobs, T. ^{[1
]}

Clemens, C. ^{[1
]}

Wunder, M. ^{[1
]}

Ruger, M. ^{[1
]}

Schulz, T. W. ^{[1
]}

机构：

[1] Boehringer Ingelheim Pharma GmbH & Co KG, Dept Biopharmaceut Proc Sci, Upstream Dev, Cell Culture Technol, Biberach, Germany

来源：

CELLS AND CULTURE | 2010年 / 4卷

关键词：

Mammalian cell cultivation; Oxygen uptake rate (OUR); Quadrupole mass spectrometry; Respiratory quotient RQ;

D O I：

10.1007/978-90-481-3419-9_118

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this investigation oxygen uptake rates (OUR) of three fed-batch processes are studied in large scale (2000 L, process A and B) and in small scale (40 L, process C) using on-line off-gas quadrupole mass spectrometry. The OUR profiles are in good agreement with typical culture parameters such as viable cell concentration, product yields, and the stoichiometric ratio. YO2/Glucose of oxygen uptake to glucose consumption. Theoretically 1 mol glucose requires 6 mol oxygen for complete oxidation under ideal culture conditions. Then glucose is effectively utilized and build-up of metabolic by-products such as lactate is minimized. By this means increased monoclonal antibody (mAb) product yields are possible.

引用

页码：683 / 687

页数：5

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