Development and pre-validation of an in vitro transactivation assay for detection of (anti)androgenic potential compounds using 22Rv1/MMTV cells

被引：14

作者：

Sun, Seunghan ^{[1
]}

Park, Eun-Jung ^{[2
]}

Choi, Yun-Ho ^{[1
]}

Lee, Hee-Seok ^{[2
]}

Ahn, Byung Yoon ^{[1
]}

Dong, Mi-Sook ^{[1
]}

机构：

[1] Korea Univ, Sch Life Sci & Biotechnol, 145 Anam Ro, Seoul 136701, South Korea

[2] Natl Inst Food & Drug Safety Evaluat, Food Safety Risk Assessment Div, Chungcheongbuk Do 363700, South Korea

来源：

REPRODUCTIVE TOXICOLOGY | 2016年 / 60卷

关键词：

Androgenic and anti-androgenic endocrine disruptors; Androgen receptor-mediated; transcriptional activation assay; 22Rv1/MMTV cell line; Endocrine disruptor screening; ENDOCRINE-DISRUPTING CHEMICALS; REPORTER GENE ASSAYS; ANDROGEN RECEPTOR; LINE;

D O I：

10.1016/j.reprotox.2016.02.006

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The endocrine-disrupting effects of androgenic signaling play crucial roles in several androgen-related diseases. In attempting to develop an in vitro cell line to be used in androgen receptor (AR)-mediated reporter gene assays, we developed a stable 22Rvl/MMTV cell line, which is a human prostate cancer cell line that endogenously expresses functional AR, to evaluate AR -mediated transcriptional activation (TA). Using 22Rv1/MMTV cells, we established and optimized a test protocol for the AR-TA assay and validated the proposed assay using 20 compounds recommended by the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM). All the performance parameters for agonist and antagonist assays were 91-100% comparable between the 22Rv1/MMTV assay and the ICCVAM report. In conclusion, the AR-TA assay using 22Rvl/MMTV cells might be a quick and relatively inexpensive method for screening large numbers of chemicals for their potential to activate or inhibit AR-mediated gene transcription. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：156 / 166

页数：11

共 3 条

[1] Characterisation and validation of an in vitro transactivation assay based on the 22Rv1/MMTV_GR-KO cell line to detect human androgen receptor agonists and antagonists
Park, Yooheon
Jung, Da-Woon
Milcamps, Anne
Takeyoshi, Masahiro
Jacobs, Miriam N.
Houck, Keith A.
Ono, Atsushi
Bovee, Toine F. H.
Browne, Patience
Delrue, Nathalie
Kang, YunSook
Lee, Hee-Seok
FOOD AND CHEMICAL TOXICOLOGY, 2021, 152
[2] Characterisation of gene expression patterns in 22RV1 cells for determination of environmental androgenic/antiandrogenic compounds
Hartel, A
Didier, A
Pfaffl, MW
Meyer, HHD
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2003, 84 (2-3): : 231 - 238
[3] A Flow Cytometry Based In Vitro Micronucleus Assay in TK6 Cells-Validation Using Early Stage Pharmaceutical Development Compounds
Lukamowicz, Magdalena
Woodward, Katherine
Kirsch-Volders, Micheline
Suter, Willi
Elhajouji, Azeddine
ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 2011, 52 (05) : 363 - 372

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