Germplasm lines are the most important genetic resources for cultivar development. ARS84-27 (Reg. no. GP-212, PI 693601), ARS98-31 (Reg. no. GP-213, PI 693602), and ARS10-82 (Reg. no. GP-214, PI 693603) are three high-yield and good malting quality two-rowed spring malting barley (Hordeum vulgare L.) germplasm lines developed by the USDA-ARS breeding program at Aberdeen, ID. All three germplasm lines were evaluated in 27 irrigated and 14 dryland trials. ARS10-82 had significantly higher yield than all the checks, and ARS98-31 and ARS84-27 were competitive with the best check cultivar 'Voyager' (Busch Agricultural Resources). Malting evaluation results showed acceptable quality profiles with specific quality traits including high extract, low protein content, and low beta-glucan for the years tested. They are all classified as moderately resistant to Fusarium head blight infection with low deoxynivalenol (DON) levels.