Novel polymorphic region of the rpoB gene containing Mycobacterium species-specific sequences and its use in identification of mycobacteria

被引：45

作者：

Lee, H

Bang, HE

Bai, GH

Cho, SN

机构：

[1] Yonsei Univ, Coll Med, Dept Microbiol, Seoul 120752, South Korea

[2] Yonsei Univ, Coll Med, Brain Korea 21 Project Med Sci, Seoul 120752, South Korea

[3] Korea Inst TB, Dept Microbiol, Seoul 137140, South Korea

[4] Int Vaccine Inst, Seoul 151600, South Korea

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2003年 / 41卷 / 05期

关键词：

D O I：

10.1128/JCM.41.5.2213-2218.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Sequence analysis of a specific region of the mycobacterium rpoB gene in 35 mycobacterial strains representing 26 different mycobacterial species of clinical importance showed that there exists a highly polymorphic region. Based on the sequences of the polymorphic region, the oligonucleotide probes of 14 mycobacterial species with relatively high clinical importance were designed and shown to be specific to their corresponding mycobacterial species by dot blot hybridization. The results showed that the probes designed in this study are highly specific to each mycobacterial species, which suggests that these sequences may be useful for the species identification of mycobacteria.

引用

页码：2213 / 2218

页数：6

共 41 条

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