Evaluation of LightCycler PCR for implementation of laboratory diagnosis of herpes simplex virus infections

被引：61

作者：

Espy, MJ ^{[1
]}

Ross, TK ^{[1
]}

Teo, R ^{[1
]}

Svien, KA ^{[1
]}

Wold, AD ^{[1
]}

Uhl, JR ^{[1
]}

Smith, TF ^{[1
]}

机构：

[1] Mayo Clin & Mayo Fdn, Div Clin Microbiol, Rochester, MN 55905 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2000年 / 38卷 / 08期

关键词：

D O I：

10.1128/JCM.38.8.3116-3118.2000

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Five hundred specimens (288 genital, 192 dermal, and 20 ocular) were extracted by technologists, and the DNA was assayed by LightCycler PCR (DNA polymerase and thymidine kinase [TK] gene targets) and by conventional tube and shell vial cell culture. One hundred fifty-eight confirmed (by cell culture and TK target PCR) positive and LightCycler-positive specimens were detected during the first 30 PCR cycles. LightCycler PCR-positive results for cycles 31 to 45 (39 of 67 [58.2%]) required confirmation by another PCR target (TK). LightCycler PCR is more sensitive (n = 197; 23.1%) than cell cultures (n = 150) for the routine laboratory detection of herpes simplex virus infections.

引用

页码：3116 / 3118

页数：3

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