Ratiometric Fluorescent Probe for the Detection of Glutathione in Living Cells

被引：5
|
作者：
Yang Runjie [1 ]
Tang Yao [1 ]
Zhu Weiping [1 ]
机构：
[1] E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China
来源：
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE | 2016年 / 37卷 / 04期
基金：
中国国家自然科学基金;
关键词：
Fluorescent probe; Glutathione; Intracellular imaging; CYSTEINE; SHIFT;
D O I：
10.7503/cjcu20150725
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A fluorescent probe( 4) for the detection of biothiols was designed and synthesized and its proper. ties for labeling glutathione was investigated. After reaction with glutathione in 2-[ 4-( 2-hydroxyethyl)-1- piperazinyl] ethanesulfonic acid( HEPES) buffer, the color of solution changed from light yellow to pink, which could be detected by naked eyes. Meanwhile, the fluorescence was enhanced at 608 nm. Probe 4 was useful for measuring glutathione at concentrations ranging from 1. 6x10(-5) to 2x10(-4) mol/L with a detection limit of 8.9x10(-7) mol/L. Besides, probe 4 was sensitive to glutathione in MCF-7 cells, which emitted red fluorescence when it was incubated with MCF-7 cells.
引用
收藏
页码：643 / 647
页数：5
相关论文
共 22 条
  • [1] Synthesis of new 2-substituted 2,5-dihydrofuranone derivatives and their chemical transformations
    Avetisyan, A. A.
    Alvandzhyan, A. G.
    Avetisyan, K. S.
    [J]. RUSSIAN JOURNAL OF ORGANIC CHEMISTRY, 2009, 45 (12) : 1871 - 1872
  • [2] Carmel R., 2001, HOMOCYSTEINE HLTH DI, P12
  • [3] Regulation of gene expression by reactive oxygen
    Dalton, TD
    Shertzer, HG
    Puga, A
    [J]. ANNUAL REVIEW OF PHARMACOLOGY AND TOXICOLOGY, 1999, 39 : 67 - 101
  • [4] Glutathione in cancer biology and therapy
    Estrela, JM
    Ortega, A
    Obrador, E
    [J]. CRITICAL REVIEWS IN CLINICAL LABORATORY SCIENCES, 2006, 43 (02) : 143 - 181
  • [5] A highly selective ratiometric near-infrared fluorescent cyanine sensor for cysteine with remarkable shift and its application in bioimaging
    Guo, Zhiqian
    Nam, SeongWon
    Park, Sungsu
    Yoon, Juyoung
    [J]. CHEMICAL SCIENCE, 2012, 3 (09) : 2760 - 2765
  • [6] A colorimetric and red emissive fluorescent probe for cysteine and its application in bioimaging
    Hou, Ji-Ting
    Yang, Jin
    Li, Kun
    Yu, Kang Kang
    Yu, Xiao-Qi
    [J]. SENSORS AND ACTUATORS B-CHEMICAL, 2015, 214 : 92 - 100
  • [7] An ESIPT based fluorescent probe for highly selective and ratiometric detection of periodate
    Huang, Chusen
    Jia, Ti
    Yu, Congjun
    Zhang, Amin
    Jia, Nengqin
    [J]. BIOSENSORS & BIOELECTRONICS, 2015, 63 : 513 - 518
  • [8] A Highly Selective OFF-ON Red-Emitting Phosphorescent Thiol Probe with Large Stokes Shift and Long Luminescent Lifetime
    Ji, Shaomin
    Guo, Huimin
    Yuan, Xiaolin
    Li, Xiaohuan
    Ding, Haidong
    Gao, Peng
    Zhao, Chunxia
    Wu, Wenting
    Wu, Wanhua
    Zhao, Jianzhang
    [J]. ORGANIC LETTERS, 2010, 12 (12) : 2876 - 2879
  • [9] Li HL, 2010, CHEM J CHINESE U, V31, P1725
  • [10] A near-infrared colorimetric fluorescent chemodosimeter for the detection of glutathione in living cells
    Li, Meng
    Wu, Xumeng
    Wang, Yao
    Li, Yongsheng
    Zhu, Weihong
    James, Tony D.
    [J]. CHEMICAL COMMUNICATIONS, 2014, 50 (14) : 1751 - 1753
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