Multiplex ligation-dependent probe amplification (MLPA) genotyping assay for mouse models of down syndrome

被引：7
|
作者：
Hewitt, Chelsee Ann
Carmichael, Catherine Louise
Wilkins, Ella Jane
Cannon, Ping Zhi Fang
Pritchard, Melanie April
Scott, Hamish Steele
机构：
[1] Walter & Eliza Hall Inst Med Res, Melbourne, Vic 3050, Australia
[2] Univ Melbourne, Dept Med Biol, Parkville, Vic 3052, Australia
[3] Monash Univ, Monash Inst Med Res, Clayton, Vic 3168, Australia
来源：
FRONTIERS IN BIOSCIENCE-LANDMARK | 2007年 / 12卷
关键词：
mouse; models; Down syndrome; Ts65Dn; genotyping assay; multiplex; ligation-dependent probe; amplification; MLPA;
D O I：
10.2741/2291
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The Ts65Dn mouse is the most widely investigated segmentally trisomic mouse model of Down syndrome. Quantitative PCR based methods are the preferred way of detecting the trisomic segment for genotyping purposes. However, identification of a 1.5 fold difference in target DNA is at the limit of detection of most quantitative PCR based methods, and in practice this can lead to difficulties in assigning genotypes. We report a 100% accurate multiplex ligation-dependent probe amplification (MLPA) assay for genotyping the Ts65Dn mouse that is also applicable to all other segmentally trisomic mouse models of Down syndrome.
引用
收藏
页码：3010 / 3016
页数：7
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