Chemically Induced Cardiomyogenesis of Mouse Embryonic Stem Cells

被引：14
|
作者：
Berkessel, Albrecht [1 ]
Seelig, Bianca [1 ]
Schwengberg, Silke [2 ]
Hescheler, Juergen [3 ,4 ]
Sachinidis, Agapios [3 ,4 ]
机构：
[1] Univ Cologne, Dept Chem, D-50939 Cologne, Germany
[2] Axiogenesis AG, D-50829 Cologne, Germany
[3] Univ Cologne CMMC, Ctr Physiol & Pathophysiol, Inst Neurophysiol, D-50931 Cologne, Germany
[4] Univ Cologne CMMC, Ctr Mol Med, D-50931 Cologne, Germany
来源：
CHEMBIOCHEM | 2010年 / 11卷 / 02期
关键词：
cardiomyogenesis; cells; cinchona alkaloids; screening; stem cells; DYNAMIC KINETIC RESOLUTION; DIFFERENTIATION; ORGANOCATALYSTS; CARDIOMYOCYTES; IDENTIFICATION; MOLECULES; SURVIVAL; ATRIAL;
D O I：
10.1002/cbic.200900345
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A transgenic murine embryonic stem (ES) cell lineage expressing enhanced green fluorescent protein (EGFP) under the control of alpha-myosine heavy chain (alpha-MHC) promoter (p alpha-MHC-EGFP) was used to investigate the effects of (thio)urea and cinchona alkaloid derivatives on cardiomyogenesis. The screening of the compounds yielded cardiomyogenesis inducing substances with good (IV-5, V-4) to very good activities (II-16, IV-8), as determined by a 50 to 80% increase in the EGFP fluorescence compared to untreated cells. Time-dependent screening approaches in which compounds were added at different developmental stages of the ES cells appeared to be of limited suitability for the identification of potential cellular targets.
引用
收藏
页码：208 / 217
页数：10
相关论文
共 50 条
  • [1] SMALL MOLECULES THAT PROMOTE CARDIOMYOGENESIS IN MOUSE EMBRYONIC STEM CELLS
    Kim, B.
    Oh, S. W.
    Lee, M. N.
    Ha, J. R.
    Jeon, H. J.
    Son, S. K.
    Lee, M. R.
    Park, J. K.
    Yoo, J. Y.
    Jeong, S. J.
    Jung, I. H.
    Lee, S. N.
    Oh, G.
    [J]. ATHEROSCLEROSIS SUPPLEMENTS, 2008, 9 (01) : 59 - 59
  • [2] Nitric oxide facilitates cardiomyogenesis in mouse embryonic stem cells
    Kanno, S
    Kim, PKM
    Sallam, K
    Lei, J
    Billiar, TR
    Shears, LL
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (33) : 12277 - 12281
  • [3] Opioid receptors and opioid peptides in the cardiomyogenesis of mouse embryonic stem cells
    Sinova, Romana
    Kudova, Jana
    Nesporova, Kristina
    Karel, Sergej
    Sulakova, Romana
    Velebny, Vladimir
    Kubala, Lukas
    [J]. JOURNAL OF CELLULAR PHYSIOLOGY, 2019, 234 (08) : 13209 - 13219
  • [4] Human embryonic stem cells for cardiomyogenesis
    Habib, Manhal
    Caspi, Oren
    Gepstein, Lior
    [J]. JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 2008, 45 (04) : 462 - 474
  • [5] hhLIM is involved in cardiomyogenesis of embryonic stem cells
    Bin Zheng
    Jin-Kun Wen
    Mei Han
    [J]. Biochemistry (Moscow), 2006, 71 : S71 - S76
  • [6] hhLIM is involved in cardiomyogenesis of embryonic stem cells
    Zheng, B
    Wen, JK
    Han, M
    [J]. BIOCHEMISTRY-MOSCOW, 2006, 71 (Suppl 1) : S71 - S76
  • [7] HIF-1alpha Deficiency Attenuates the Cardiomyogenesis of Mouse Embryonic Stem Cells
    Kudova, Jana
    Prochazkova, Jirina
    Vasicek, Ondrej
    Perecko, Tomas
    Sedlackova, Miroslava
    Pesl, Martin
    Pachernik, Jiri
    Kubala, Lukas
    [J]. PLOS ONE, 2016, 11 (06):
  • [8] Small molecules that induce cardiomyogenesis in embryonic stem cells
    Wu, X
    Ding, S
    Ding, G
    Gray, NS
    Schultz, PG
    [J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (06) : 1590 - 1591
  • [9] Overexpression of hhlim enhances cardiomyogenesis of embryonic stem cells
    Zheng, B
    Wen, JK
    Han, M
    Zhou, AR
    [J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, 2004, 31 (06) : 506 - 511
  • [10] Mouse mutants from chemically mutagenized embryonic stem cells
    Munroe, RJ
    Bergstrom, RA
    Zheng, QY
    Libby, B
    Smith, R
    John, SWM
    Schimenti, KJ
    Browning, VL
    Schimenti, JC
    [J]. NATURE GENETICS, 2000, 24 (03) : 318 - 321
12345