Real-time monitoring the efficacy of 7-hydroxycoumarin to cells cultured on microfluidics in different extracellular pH environments by chip-mass spectrometry

被引：8

作者：

Han, Shuang ^{[1
,2
]}

Zhang, Pengbo ^{[1
]}

Lin, Haifeng ^{[2
]}

Li, Haifang ^{[2
]}

Guo, Yanli ^{[3
]}

Xu, Ning ^{[2
]}

Lin, Jin-Ming ^{[2
]}

机构：

[1] Xinxiang Med Univ, Sch Publ Hlth, Xinxiang 453003, Henan, Peoples R China

[2] Tsinghua Univ, Dept Chem,Minist Educ, Beijing Key Lab Microanalyt Methods & Instrumenta, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Beijing 100084, Peoples R China

[3] Shimadzu China Co, Beijing Branch, China Innovat Ctr, Beijing 100020, Peoples R China

来源：

TALANTA | 2022年 / 243卷

基金：

中国国家自然科学基金; 国家重点研发计划;

关键词：

Microfluidics chip; Mass spectrometry; Drug metabolism; Extracellular environment; DRUG-RESISTANCE; CANCER; TUMOR; METABOLISM; COUMARIN;

D O I：

10.1016/j.talanta.2022.123331

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The extracellular microenvironments play a key role in tumor metabolism. To online dynamic monitoring the efficacy of 7-hydroxycoumarin (7-OHC) to cells cultured on microfluidics in acidic microenvironment, we developed an integrated multi-channel chip-mass spectrometry system. This system has six drug-loading units, cell culture chamber, metabolite collection, filtration, HPLC separation and MS detection. The cells in each microchannel will be incubated with continuous flow of culture medium, metabolites will be collected by the fixed card slot, automatic sampling needle will be precise positioned and sampled. Through this new system, the 7-hydroxycoumarin-sulfonate (7-OHC-sulfonate) and 7-hydroxycoumarin-glucuronide (7-OHC-glucuronide) can be determined in real-time. The results revealed that the addition of lactic acid promoted the formation of inactive 7-OHC-sulfonate and 7-OHC-glucuronide metabolites. Besides, acidic extracellular environment amplified cancer cell proliferation, indicating the anticancer effect of 7-OHC was weakened by low extracellular pH.

引用

页数：6

共 3 条

[1] Real-time monitoring system for evaluating the acid-producing activity of oral squamous cell carcinoma cells at different environmental pH
Morishima, Hiromitsu
Washio, Jumpei
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Shinohara, Yuta
Takahashi, Tetsu
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[J]. SCIENTIFIC REPORTS, 2017, 7
[2] Real-time monitoring system for evaluating the acid-producing activity of oral squamous cell carcinoma cells at different environmental pH
Hiromitsu Morishima
Jumpei Washio
Jun Kitamura
Yuta Shinohara
Tetsu Takahashi
Nobuhiro Takahashi
[J]. Scientific Reports, 7
[3] Towards monitoring real-time cellular response using an integrated microfluidics-matrix assisted laser desorption ionisation/nanoelectrospray ionisation-ion mobility-mass spectrometry platform
Enders, J. R.
Marasco, C. C.
Kole, A.
Nguyen, B.
Sevugarajan, S.
Seale, K. T.
Wikswo, J. P.
McLean, J. A.
[J]. IET SYSTEMS BIOLOGY, 2010, 4 (06) : 416 - 427

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