Adventitious shoot regeneration from leaf explants of kakrol cultured in vitro

Adventitious shoot bud differentiation occurred from the petiolar cut ends of leaf explants of Momordica dioica (Roxb.) Willd.. cultured on Murashige and Skoog medium supplemented with different concentrations and combinations of BA and NAA. The best response of shoot proliferation was obtained in medium supplemented with 2.0 mg/l BA and 0.2 mg/l NAA. Direct and indirect (via callus) shoot bud differentiation were observed. After 3 weeks of culture, calli were transferred onto the MS medium supplemented with aforesaid growth regulator for bud regeneration. Rooting was induced with 1.0 mg/l BA and plantlets were survived successfully to field condition. This is the first description of adventitious bud regeneration from leaf explants of kakrol.