Electrocatalytic Detection and Quantification of Nitazoxanide Based on Graphene-Polyaniline (Grp-Pani) Nanocomposite Sensor

An ultrasensitive voltammetric sensor based on the synergistic effect of graphene (Grp)-Polyaniline (Pani) hybrid film for electrochemical sensing of antiprotozoal nitazoxanide has been developed. The electrocatalytic performance and morphology of fabricated hybrid film sensor were characterized by square wave voltammetry (SWV), scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). The influence of various parameters such as pH, effect of scan rate, and loading of Pani-Grp hybrid film onto GCE on electroanalytical performance of the developed sensor was investigated. Under optimized conditions, reduction peak current was linear over the wide concentration range from 10-70 mu g/mL with correlation coefficient of 0.998. The lower limit of detection (LOD) and the lower limit of quantification (LOQ) were found to be 2.2 mu g/mL and 6.9 mu g/mL. A novel electrocatalytic activity and the influence of hybrid film results in good characteristics such as high electroactive surface area, excellent electrochemical performance, anti-interferent ability, good stability, fast response, good reproducibility and long-term stability which are the promising features of the biosensing applications. (C) 2014 The Electrochemical Society. All rights reserved.