Nocardioides insulae sp nov., isolated from soil

A Gram-positive, rod-shaped or coccoid bacterial strain, DS-51(T), was isolated from a soil in Dokdo, Korea, and its taxonomic position was investigated by using a polyphasic approach. Strain DS-51(T) grew optimally at pH 8.0 and 30 degrees C without NaCl. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain DS-51(T) forms a distinct line of descent within the radiation enclosed by the genus Nocardioides. The chemotaxonomic properties of strain DS-51(T) were consistent with those of the genus Nocardioides: the cell-wall pepticloglycan type was based on LL-2,6-diaminopimelic acid, MK-8(H-4) was the predominant menaquinone and iso-C-16:0 was the major fatty acid. The DNA G + C content was 71.1 mol%, The 16S rRNA gene sequence of strain DS-51(T) had similarity levels of 92.5-95.1% with the sequences of the type strains of Nocardioides species. Strain DS-51(T) could be distinguished from other Nocardioides species by differences in some phenotypic characteristics. On the basis of the phenotypic, chemotaxonomic and phylogenetic data, strain DS-51(T) represents a novel species of the genus Nocardioides, for which the name Nocardioides insulae sp. nov. is proposed. The type strain is DS-51(T) (= KCTC 19180(T) = DSM 17944(T)).