Purification and characterisation of an alkaline protease used in tannery industry from Bacillus licheniformis

被引：25
|
作者：
Tang, XM
Lakay, FM
Shen, W
Shao, WL
Fang, HY
Prior, BA
Wang, ZX
Zhuge, J
机构：
[1] Univ Stellenbosch, Dept Microbiol, ZA-7602 Matieland, South Africa
[2] So Yangtze Univ, Key Lab Ind Biotechnol, Minist Educ, Res Ctr Ind Microbiol, Wuxi 214036, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 18期
关键词：
alkaline protease; Bacillus licheniformis; characterisation; purification;
D O I：
10.1023/B:BILE.0000045642.19299.3f
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An extracellular alkaline protease produced by Bacillus licheniformis AP-1 was purified 76-fold, yielding a single 28 kDa band on SDS-PAGE. It was optimally active at pH 11 and at 60degreesC (assayed over 10 min). The protease was completely inhibited by phenylmethylsulfonyl fluoride and diodopropyl fluorophosphate, with little increase upon Ca2+ and Mg2+ addition.
引用
收藏
页码：1421 / 1424
页数：4
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