Development and characterization of twenty-three microsatellite markers for the purpleback flying squid (Symplectoteuthis oualaniensis)

被引：3
|
作者：
Lin, Lin [1 ,2 ]
Li, Chunhou [1 ,2 ]
Chen, Zuozhi [1 ,2 ]
Xu, Shannan [1 ,2 ]
Liu, Yong [1 ,2 ]
机构：
[1] Chinese Acad Fishery Sci, Minist Agr, Key Lab Exploitat & Utilizat Marine Fisheries Res, Guangzhou, Guangdong, Peoples R China
[2] Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou, Guangdong, Peoples R China
来源：
CONSERVATION GENETICS RESOURCES | 2015年 / 7卷 / 01期
关键词：
Symplectoteuthis oualaniensis; Purpleback flying squid; Microsatellite; Genetic diversity; SOFTWARE;
D O I：
10.1007/s12686-014-0318-1
中图分类号：
X176 [生物多样性保护];
学科分类号：
090705 ;
摘要：
The purpleback flying squid (Symplectoteuthis oualaniensis) is a pelagic squid that is facing the serious threat of large-scale commercial exploitation in the South China Sea. In this study, microsatellites were developed for the first time in this species using biotinylated probes for hybridization and library screening. Twenty-three polymorphic microsatellite markers were isolated and characterized in a wild population. The number of alleles varied from 5 to 28, and the observed and expected heterozygosity ranged from 0.220 to 0.867 and 0.374 to 0.958, respectively. Significant deviations from Hardy-Weinberg equilibrium values were detected in six loci. These markers will facilitate further studies in conservation of genetic diversity and sustainable utilization of S. oualaniensis.
引用
收藏
页码：161 / 163
页数：3
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