Improved xylitol production by expressing a novel D-arabitol dehydrogenase from isolated Gluconobacter sp JX-05 and co-biotransformation of whole cells

被引：20

作者：

Qi, Xianghui ^{[1
]}

Zhang, Huanhuan ^{[1
]}

Magocha, Tinashe Archbold ^{[1
]}

An, Yingfeng ^{[2
]}

Yun, Junhua ^{[1
]}

Yang, Miaomiao ^{[1
]}

Xue, Yanbo ^{[1
]}

Liang, Shuhua ^{[3
]}

Sun, Wenjing ^{[1
]}

Cao, Zheng ^{[4
]}

机构：

[1] Jiangsu Univ, Sch Food & Biol Engn, 301 Xuefu Rd, Zhenjiang 212013, Jiangsu, Peoples R China

[2] Shenyang Agr Univ, Coll Biosci & Biotechnol, 120 Dongling Rd, Shenyang 110161, Liaoning, Peoples R China

[3] Nanning Bioclone Biotechnol Co Ltd, 5 Keyuan Rd, Nanning 530001, Guangxi, Peoples R China

[4] Univ Calif Los Angeles, Dept Chem & Biochem, 611 Charles E Young Dr E, Los Angeles, CA 90095 USA

来源：

BIORESOURCE TECHNOLOGY | 2017年 / 235卷

基金：

中国国家自然科学基金;

关键词：

Gluconobacter sp; D-Arabitol dehydrogenase; Characteristic; Biotransformation; Whole cell; SHORT-CHAIN DEHYDROGENASES/REDUCTASES; ESCHERICHIA-COLI; BIOTECHNOLOGICAL PRODUCTION; FUNCTIONAL ASSIGNMENTS; MANNITOL DEHYDROGENASE; OXYDANS; STRAINS; GENE; PURIFICATION; SEQUENCE;

D O I：

10.1016/j.biortech.2017.03.107

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

In the present study, a novel ardh gene encoding D-arabitol dehydrogenase (ArDH) was cloned and expressed in Escherichia coli from a new isolated strain of Gluconobacter sp. JX-05. Sequence analysis revealed that ArDH containing a NAD(P)-binding motif and a classical active site motif belongs to the short-chain dehydrogenase family. Subsequently, the optimal pH and temperature, specific activities and kinetic parameter of ArDH were determined. In the co-biotransformation by the whole cells of BL21-ardh and BL21-xdh, 26.1 g/L xylitol was produced from 30 g/L D-arabitol in 22 h with a yield of 0.87 g/g. The xylitol production was increased by more than two times as compared with that of Gluconobacter sp. alone, and was improved 10.1% than that of Gluconobacter sp. mixed with BL21- xdh. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：50 / 58

页数：9

共 6 条

[1] Production of xylitol by expressing xylitol dehydrogenase and alcohol dehydrogenase from Gluconobacter thailandicus and co-biotransformation of whole cells
Zhang, Huanhuan
Yun, Junhua
Zabed, H.
Yang, Miaomiao
Zhang, Guoyan
Qi, Yilin
Guo, Qi
Qi, Xianghui
[J]. BIORESOURCE TECHNOLOGY, 2018, 257 : 223 - 228
[2] Cloning of the xylitol dehydrogenase gene from Gluconobacter oxydans and improved production of xylitol from D-arabitol
Sugiyama, M
Suzuki, S
Tonouchi, N
Yokozeki, K
[J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2003, 67 (03) : 584 - 591
[3] Novel enzymatic method for the production of xylitol from D-arabitol by Gluconobacter oxydans
Suzuki, S
Sugiyama, M
Mihara, Y
Hashiguchi, K
Yokozeki, K
[J]. BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2002, 66 (12) : 2614 - 2620
[4] Improved Xylitol Production from D-Arabitol by Enhancing the Coenzyme Regeneration Efficiency of the Pentose Phosphate Pathway in Gluconobacter oxydans
Li, Sha
Zhang, Jinliang
Xu, Hong
Feng, Xiaohai
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2016, 64 (05) : 1144 - 1150
[5] Construction and co-expression of plasmid encoding xylitol dehydrogenase and a cofactor regeneration enzyme for the production of xylitol from D-arabitol
Zhou, Peng
Li, Sha
Xu, Hong
Feng, Xiaohai
Ouyang, Pingkai
[J]. ENZYME AND MICROBIAL TECHNOLOGY, 2012, 51 (02) : 119 - 124
[6] Production of 1,3-propanediol using a novel 1,3-propanediol dehydrogenase from isolated Clostridium butyricum and co-biotransformation of whole cells
Yun, Junhua
Yang, Miaomiao
Magocha, Tinashe A.
Zhang, Huanhuan
Xue, Yanbo
Zhang, Guoyan
Qi, Xianghui
Sun, Wenjing
[J]. BIORESOURCE TECHNOLOGY, 2018, 247 : 838 - 843

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