Synthesis of methylketone containing nucleoside triphosphates for RNA labelling

被引：26
|
作者：
Trévisiol, E
Defrancq, E
Lhomme, J
Laayoun, A
Cros, P
机构：
[1] Univ Grenoble 1, Etud Dynam & Struct Select Lab, CNRS, UMR 5616, F-38041 Grenoble 9, France
[2] BioMerieux, Lab Sondes Nucl, F-69280 Marcy Etoile, France
来源：
TETRAHEDRON | 2000年 / 56卷 / 35期
关键词：
fluorescence; labelling; ketone; nucleotides; phosphorylation;
D O I：
10.1016/S0040-4020(00)00577-9
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
The three nucleoside triphosphates 1, 2 and 3 bearing a linker with a terminal methylketone group were prepared for incorporation into RNA fragments and post-labelling by the fluorescein derivative 4 containing an aminooxy group. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码：6501 / 6510
页数：10
相关论文
共 50 条
  • [1] Synthesis of Dinucleoside Triphosphates Containing Antiviral Nucleoside
    Liu, Rong
    Gongb, Shan-Shan
    PROCEEDINGS OF THE 2016 INTERNATIONAL CONFERENCE ON BIOLOGICAL ENGINEERING AND PHARMACY (BEP 2016), 2016, 3 : 215 - 217
  • [2] Convenient synthesis of nucleoside 5′-triphosphates for RNA transcription
    Caton-Williams, Julianne
    Lin, Lina
    Smith, Matthew
    Huang, Zhen
    CHEMICAL COMMUNICATIONS, 2011, 47 (28) : 8142 - 8144
  • [3] Synthesis of nucleoside triphosphates that contain an aminooxy function for "post-amplification labelling"
    Trévisiol, E
    Defrancq, E
    Lhomme, J
    Laayoun, A
    Cros, P
    EUROPEAN JOURNAL OF ORGANIC CHEMISTRY, 2000, 2000 (01) : 211 - 217
  • [4] RELATIONSHIP BETWEEN CONCENTRATION OF NUCLEOSIDE TRIPHOSPHATES AND RATE OF SYNTHESIS OF RNA
    BECK, C
    INGRAHAM, J
    MAALOE, O
    NEUHARD, J
    JOURNAL OF MOLECULAR BIOLOGY, 1973, 78 (01) : 117 - 121
  • [5] Modified nucleoside triphosphates:: Synthesis and applications for RNA in vitro selection
    Schoetzau, T
    Langner, J
    Moyroud, E
    Röhl, I
    Klussmann, S
    Vonhoff, S
    NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, 2003, 22 (5-8): : 1293 - 1296
  • [6] INHIBITORY EFFECTS OF NUCLEOSIDE TRIPHOSPHATES ON NUCLEOLAR RNA-SYNTHESIS
    NAGAMINE, Y
    MIZUNO, D
    NATORI, S
    JOURNAL OF BIOCHEMISTRY, 1979, 85 (03): : 839 - 845
  • [7] Synthesis of morpholine nucleoside triphosphates
    Abramova, TV
    Bakharev, PA
    Vasilyeva, SV
    Silnikov, VN
    TETRAHEDRON LETTERS, 2004, 45 (22) : 4361 - 4364
  • [8] CHEMICAL SYNTHESIS OF NUCLEOSIDE TRIPHOSPHATES
    OTT, DG
    KERR, VN
    HANSBURY, E
    HAYES, FN
    ANALYTICAL BIOCHEMISTRY, 1967, 21 (03) : 469 - &
  • [9] SYNTHESIS OF CIS-2-PENTENE-CONTAINING NUCLEOSIDE ANALOGS AND THEIR TRIPHOSPHATES
    MITSNER, BI
    KOCHETKOVA, MV
    FILIPPOV, DV
    TSYTOVICH, AV
    DYATKINA, NB
    MOLECULAR BIOLOGY, 1993, 27 (01) : 105 - 111
  • [10] Synthesis and biological evaluation of nucleoside triphosphates incorporating an oxyamino function for "post-amplification labelling"
    Trévisiol, E
    Defrancq, E
    Lhomme, J
    Laayoun, A
    Hoang, A
    Besème, F
    Cros, P
    NUCLEOSIDES & NUCLEOTIDES, 1999, 18 (4-5): : 979 - 980
12345