The Coordinated Upregulated Expression of Genes Involved in MEP, Chlorophyll, Carotenoid and Tocopherol Pathways, Mirrored the Corresponding Metabolite Contents in Rice Leaves during De-Etiolation

被引：6

作者：

Jin, Xin ^{[1
,2
,3
]}

Baysal, Can ^{[3
]}

Drapal, Margit ^{[4
]}

Sheng, Yanmin ^{[5
]}

Huang, Xin ^{[3
]}

He, Wenshu ^{[3
]}

Shi, Lianxuan ^{[6
]}

Capell, Teresa ^{[3
]}

Fraser, Paul D. ^{[4
]}

Christou, Paul ^{[3
,7
]}

Zhu, Changfu ^{[3
,5
]}

机构：

[1] Gansu Agr Univ, Gansu Prov Key Lab Aridland Crop Sci, Lanzhou 730070, Peoples R China

[2] Gansu Agr Univ, Coll Life Sci & Technol, Lanzhou 730070, Peoples R China

[3] Univ Lleida Agrotecnio CERCA Ctr, Dept Plant Prod & Forestry Sci, Ave Alcalde Rovira Roure 191, Lleida 25198, Spain

[4] Royal Holloway Univ London, Sch Life Sci & Environm, Biochem, Egham Hill, Egham TW20 0EX, Surrey, England

[5] Changchun Normal Univ, Sch Life Sci, Changchun 130032, Peoples R China

[6] Northeast Normal Univ, Sch Life Sci, Changchun 130024, Peoples R China

[7] Catalan Inst Res & Adv Studies, ICREA, Passeig Lluis Co 23, Barcelona 08010, Spain

来源：

PLANTS-BASEL | 2021年 / 10卷 / 07期

基金：

中国国家自然科学基金;

关键词：

carotenoids; coordinated gene expression; light-upregulated genes; isoprenoids; rice (Oryza sativa L.); secondary metabolites; PHYTOENE SYNTHASE; ISOPRENOID BIOSYNTHESIS; LIGHT; ACTIVATION; TRANSCRIPT; ELEMENTS; DATABASE; ENZYME;

D O I：

10.3390/plants10071456

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Light is an essential regulator of many developmental processes in higher plants. We investigated the effect of 4-hydroxy-3-methylbut-2-enyl diphosphate reductase 1/2 genes (OsHDR1/2) and isopentenyl diphosphate isomerase 1/2 genes (OsIPPI1/2) on the biosynthesis of chlorophylls, carotenoids, and phytosterols in 14-day-old etiolated rice (Oyza sativa L.) leaves during de-etiolation. However, little is known about the effect of isoprenoid biosynthesis genes on the corresponding metabolites during the de-etiolation of etiolated rice leaves. The results showed that the levels of alpha-tocopherol were significantly increased in de-etiolated rice leaves. Similar to 1-deoxy-D-xylulose-5-phosphate synthase 3 gene (OsDXS3), both OsDXS1 and OsDXS2 genes encode functional 1-deoxy-D-xylulose-5-phosphate synthase (DXS) activities. Their expression patterns and the synthesis of chlorophyll, carotenoid, and tocopherol metabolites suggested that OsDXS1 is responsible for the biosynthesis of plastidial isoprenoids in de-etiolated rice leaves. The expression analysis of isoprenoid biosynthesis genes revealed that the coordinated expression of the MEP (2-C-methyl-D-erythritol 4-phosphate) pathway, chlorophyll, carotenoid, and tocopherol pathway genes mirrored the changes in the levels of the corresponding metabolites during de-etiolation. The underpinning mechanistic basis of coordinated light-upregulated gene expression was elucidated during the de-etiolation process, specifically the role of light-responsive cis-regulatory motifs in the promoter region of these genes. In silico promoter analysis showed that the light-responsive cis-regulatory elements presented in all the promoter regions of each light-upregulated gene, providing an important link between observed phenotype during de-etiolation and the molecular machinery controlling expression of these genes.

引用

页数：16