The present study extrapolates the assessment and characterization of a barely studied region, the Gulf of Kutch, (near Jamnagar), Gujarat, India, in terms of PAH exposure, adverse effects caused by them, and various toxicological indices showing the catastrophic effects of their elevated concentrations. Sigma PAH concentration in the site ranged from 118,280 to 1,099,410 ng g(-1) dw, with a predominance of 2-3-ring PAHs (79.09%) as compared to 4-5- and 6-ring PAHs (20.91%). The concentrations of carcinogenic PAHs were found to be between 8120 and 160,000 ng g(-1) dw, with a mean of 63,810 ng g-1 dw, which is much higher than normal acceptable values. The toxic equivalent quotient for 6CPAHs ranged from 150.47 to 26,330 ng g(-1) BaPeq, encompassing 50.63% of Sigma PAH toxicity. This toxicological profile of the present study site would be of paramount importance as it offers fresh information regarding the load of legacy pollutants such as PAHs and the inputs and methods to cope with their extremely high concentrations in less explored marine habitats.