Genome-Wide Analysis of Members of the WRKY Gene Family and Their Cold Stress Response in Prunus mume

被引:33
|
作者
Bao, Fei [1 ,2 ,3 ,4 ,5 ]
Ding, Anqi [1 ,2 ,3 ,4 ,5 ,6 ]
Cheng, Tangren [2 ,3 ,4 ,5 ]
Wang, Jia [2 ,3 ,4 ,5 ]
Zhang, Qixiang [1 ,2 ,3 ,4 ,5 ,6 ]
机构
[1] Beijing Forestry Univ, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Beijing 100083, Peoples R China
[2] Beijing Forestry Univ, Beijing Key Lab Ornamental Plants Germplasm Innov, Beijing 100083, Peoples R China
[3] Beijing Forestry Univ, Natl Engn Res Ctr Floriculture, Beijing 100083, Peoples R China
[4] Beijing Forestry Univ, Beijing Lab Urban & Rural Ecol Environm, Beijing 100083, Peoples R China
[5] Beijing Forestry Univ, Key Lab Genet & Breeding Forest Trees & Ornamenta, Minist Educ, Beijing 100083, Peoples R China
[6] Beijing Forestry Univ, Sch Landscape Architecture, Beijing 100083, Peoples R China
基金
中国国家自然科学基金;
关键词
Prunus mume; genome; WRKY genes; cold response; TRANSCRIPTION FACTOR; DROUGHT TOLERANCE; OXIDATIVE STRESS; ABSCISIC-ACID; ARABIDOPSIS; IDENTIFICATION; SUPERFAMILY; DUPLICATION; EXPRESSION; SEQUENCES;
D O I
10.3390/genes10110911
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Prunus mume, which is a rosaceous arbor with very high ornamental, edible and medical values, has a distribution that is mainly restricted by low temperature. WRKY transcription factor genes play crucial roles in the growth, development, and stress responses of plants. However, the WRKY gene family has not been characterised in P. mume. There were 58 PmWRKYs identified from genome of P. mume. They were anchored onto eight link groups and categorised into three broad groups. The gene structure and motif composition were reasonably conservative in each group. Investigation of gene duplication indicated that nine and seven PmWRKYs were arranged in tandem and segmental duplications, respectively. PmWRKYs were discriminately expressed in different tissues (i.e., roots, stems, leaves, flowers and fruits) in P. mume. The 17 cold-related candidate genes were selected based on RNA-seq data. Further, to investigate the function of PmWRKYs in low temperatures, the expression patterns under artificial cold treatments were analysed. The results showed that the expression levels of the 12 PmWRKYs genes significantly and 5 genes slightly changed in stems. In particular, the expression level of PmWRKY18 was up-regulated after ABA treatment. In addition, the spatiotemporal expression patterns of 17 PmWRKYs were analysed in winter. These results indicated that 17 PmWRKYs were potential transcription factors regulating cold resistance in P. mume.
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页数:20
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