Characterization of the complete mitochondrial genome of Centrorhynchus milvus Ward, 1950 (Acanthocephala: Polymorphida) and its phylogenetic implications

The phylum Acanthocephala is a small group of obligate parasites of animals. However, the current classifications of Acanthocephala are still under debate. Moreover, our present knowledge of the complete mitochondrial genome of this parasite group remains limited. To fill this knowledge gap, the complete mitochondrial (mt) genome of Centrorhynchus milvus Ward, 1956 (Palaeacanthocephala: Polymorphida) was firstly sequenced and determined based on specimens collected from the red kite (Milvus milvus) in Pakistan. The mitochondrial genome of C. milvus is 14,314 bp in length and contains 36 genes, including 12 protein-coding (PCGs) genes, 22 transfer RNA (tRNA) genes and ribosomal RNA (rRNA) genes (rrnL and rrnS). To elucidate the phylogenetic relationships of the four classes of Acanthocephala and the systematic position of C. milvus, phylogenetic analysis based on concatenated amino acid sequences of 12 PCGs was performed using Bayesian inference (BI). The results supported the monophyly of Archiacanthocephala and Palaeacanthocephala with strong support (BPP = 1) and also indicated that Archiacanthocephala is the sister Glade to the remaining classes of Acanthocephala (Palaeacanthocephala, Eoacanthocephala and Polyacanthocephala). However, Polyacanthocephala with only one representative species (P. caballeroi) is nested within Eoacanthocephala. Our phylogenetic analysis also confirmed C. milvus as the member of the family Centrorhynchidae with a sister relationship to C. aluconis. Our present mt genomic data are very useful for studying the molecular epidemiology, population genetics and systematics of acanthocephalans.