Monoclonal antibodies against hepatitis BS antigen: Production, characterization, and use for diagnosis

被引：2
|
作者：
Acosta, C
Baluja, I
Amores, I
Brito, A
Valdivia, I
Delhanty, A
Ventura, J
Soto, V
机构：
[1] Ctr Inmunoensayo, Lab Anticuerpos Monoclonales, C Habana, Cuba
[2] Ctr Inmunoensayo, Lab Hepatitis, C Habana, Cuba
[3] Ctr Inmunoensayo, Dept Bioterio, C Habana, Cuba
来源：
HYBRIDOMA | 2000年 / 19卷 / 03期
关键词：
D O I：
10.1089/02724570050109657
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Three different hybridoma clones secreting anti-HBsAg antibody were constructed by fusing cells of mouse myeloma line Ag8-X63 with splenocytes from BALB/c mice immunized with recombinant HBsAg and natural HBV. The monoclonal antibodies obtained were characterized immunologically, and two were used to develop UMELISA for detection of HBsAg. This monoclonal assay enabled the detection of 0.1 UPE/mL with reference to the standard of the Paul Ehrlich Institute (Frankfurt, Germany). The assay compared well with a commercially available kit (UMELISA HBsAg) and was used for detection of HBsAg in blood donors.
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收藏
页码：259 / 262
页数：4
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