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Stereoselective prostereogenic 3-oxo ester reduction mediated by a novel yeast alcohol dehydrogenase derived from Kluyveromyces marxianus CBS 6556
被引：12
|作者：
Perrone, Maria Grazia
Santandrea, Ernesto
Scilimati, Antonio
Syldatk, Christoph
机构：
[1] Univ Bari, Dipartimento Farmacochim, I-70125 Bari, Italy
[2] Univ Karlsruhe, Engler Bunte Inst, Lehrstuhl Tech Biol, D-76131 Karlsruhe, Germany
关键词：
alcohol dehydrogenase;
asymmetric bioreduction;
fermentation;
Kluyveromyces marxianus;
3-oxo esters;
D O I：
10.1002/adsc.200600379
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A yeast alcohol dehydrogenase (ADH) has been purified up to a purification factor value of 21,731-fold from Kluyveromyces marxianus CBS 6556. The purification procedure consisted of two chromatographic steps (DEAE-anion exchange and affinity chromatography). The optimal pH was 7, its optimal temperature was 40 degrees C and its co-factor was NADPH. This novel ADH efficiently mediated the reduction of 3-oxo esters with a high degree of ste-reoselectivity, providing chiral alcohols having the (S) absolute configuration at the newly formed stereogenic centre by delivering the hydride from the re-face of the prochiral carbonyl compounds.
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页码：1111 / 1118
页数：8
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