Prostaglandin E2 synthesis in human monocyte-derived dendritic cells

被引:0
|
作者
Norgauer, J
Ibig, Y
Gmeiner, D
Herouy, Y
Fiebich, BL
机构
[1] Univ Freiburg, Dept Expt Dermatol, D-79104 Freiburg, Germany
[2] Univ Freiburg, Dept Psychiat, D-79104 Freiburg, Germany
关键词
dendritic cells; cyclooxygenase; prostaglandin E-2; p38 stress-activated protein kinase;
D O I
暂无
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Prostaglandin E-2 (PGE(2)), which is generated by the enzymatic activity of cyclooxygenase-1 and -2 (COX-1/2), plays a central role in the maturation process of dendritic cells (DC). Since regulation of COX-1/2 expression in human DC is only partially understood, we addressed the expression and activity of COX-1/2 in these cells. Here we show that lipopolysaccharide (lps) induces COX-2 mRNA and protein synthesis as well as the release of PGE(2) in human interieukin-4 and granulocyte/macrophage colony-stimulating factor-differentiated monocyte-derived DC cultivated in the presence of 1% human plasma. Moreover, we found that lps induces p38 stress-activated protein kinase (p38) in these cells and inhibitors of p38 blocked lps induced COX-2 expression and activity. Our data indicate that during lps-induced maturation p38 regulates COX-2 expression and PGE(2) synthesis in DC.
引用
收藏
页码:83 / 86
页数:4
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