Extraction and amplification of authentic DNA from ancient human remains

被引：30
|
作者：
Meyer, E
Wiese, M
Bruchhaus, H
Claussen, M
Klein, A
机构：
[1] Univ Jena, Inst Legal Med, D-07743 Jena, Germany
[2] Univ Jena, Inst Human Genet & Anthropol, D-07743 Jena, Germany
来源：
FORENSIC SCIENCE INTERNATIONAL | 2000年 / 113卷 / 1-3期
关键词：
ancient DNA; molecular anthropology; amelogenin; sex determination;
D O I：
10.1016/S0379-0738(00)00220-6
中图分类号：
DF [法律]; D9 [法律]; R [医药、卫生];
学科分类号：
0301 ; 10 ;
摘要：
A total of 36 ancient human remains from 12 individuals (three tooth/bone samples each) and one sample each of three individuals from the newest time was typed in a blind study using the amelogenin sex test. Prior to this molecular sex determination the sex of the individual was determined morphologically. The success rate of the amelogenin amplifications was >90%. For every individual an unambiguous molecular sex typing result was obtained. Furthermore, the morphological and molecular sex determinations were in accordance with each other, giving evidence for the authenticity and ancient origin of the polymerase chain reaction (PCR) amplifications. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码：87 / 90
页数：4
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