Antibody catalyzed hydrolysis of a quorum sensing signal found in Gram-negative bacteria

被引：45
|
作者：
Marin, Sandra De Lamo
Xu, Yang
Meijler, Michael M.
Janda, Kim D.
机构：
[1] Skaggs Inst Chem Biol, Dept Chem, La Jolla, CA 92037 USA
[2] Scripps Res Inst, WIRM, La Jolla, CA 92037 USA
[3] Skaggs Inst Chem Biol, Dept Immunol, La Jolla, CA 92037 USA
来源：
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2007年 / 17卷 / 06期
关键词：
quorum sensing; catalytic antibodies; homoserine lactone hydrolysis; inhibitor;
D O I：
10.1016/j.bmcl.2006.12.118
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
N-(3-Oxo-acyl) homoserine lactones are used by Gram-negative bacteria to signal the establishment of specific population densities and coordinate population-wide gene expression. Herein we report the antibody-catalyzed hydrolysis of N-(3-oxo-acyl) homoserme lactone (AHL) using a reactive immunization strategy with a squaric monoester monoamide hapten. Kinetic analysis of the most efficient antibody revealed a modest kcat, with AHL hydrolysis competitively inhibited by original squaric monoester monoamide hapten. These studies suggest that antibody catalysis could provide a new avenue for blocking quorum sensing in bacteria. (c) 2007 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1549 / 1552
页数：4
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