In-plane Extended Nano-coulter Counter (XnCC) for the Label-free Electrical Detection of Biological Particles

被引：3

作者：

Zhao, Zheng ^{[1
,2
]}

Vaidyanathan, Swarnagowri ^{[1
,2
]}

Bhanja, Payel ^{[3
,4
]}

Gamage, Sachindra ^{[2
,5
]}

Saha, Subhrajit ^{[3
,4
]}

McKinney, Collin ^{[2
,6
]}

Choi, Junseo ^{[2
,6
]}

Park, Sunggook ^{[2
,6
]}

Pahattuge, Thilanga ^{[2
,5
]}

Wijerathne, Harshani ^{[2
,5
]}

Jackson, Joshua M. ^{[2
,5
]}

Huppert, Mateusz L. ^{[7
]}

Witek, Malgorzata A. ^{[2
,5
]}

Soper, Steven A. ^{[1
,2
,4
,5
,8
,9
]}

机构：

[1] Univ Kansas, Bioengn Program, Lawrence, KS 66045 USA

[2] Univ Kansas, Ctr BioModular Multiscale Syst Precis Med, Lawrence, KS 66045 USA

[3] Univ Kansas, Dept Radiat Oncol, Med Ctr, Kansas City, KS 66160 USA

[4] Univ Kansas, Med Ctr, Canc Ctr, Kansas City, KS 66160 USA

[5] Univ Kansas, Dept Chem, Lawrence, KS 66045 USA

[6] Univ N Carolina, CRITCL, Chapel Hill, NC 27515 USA

[7] Louisiana State Univ, Dept Ind & Mech Engn, Baton Rouge, LA 70803 USA

[8] BioFluidica Inc, San Diego, CA 92121 USA

[9] Univ Kansas, Dept Mech Engn, Lawrence, KS 66045 USA

来源：

ELECTROANALYSIS | 2022年 / 34卷 / 12期

基金：

美国国家卫生研究院;

关键词：

Coulter counter; nanoparticles; COVID-19; exosomes; RESISTIVE PULSE SENSOR; EXTRACELLULAR VESICLES; IONIZING-RADIATION; EXOSOMES; CELLS; SIZE; EXPOSURE; CHARGE; IDENTIFICATION; NANOPARTICLES;

D O I：

10.1002/elan.202200091

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report an in-plane extended nanopore Coulter counter (XnCC) chip fabricated in a thermoplastic via imprinting. The fabrication of the sensor utilized both photolithography and focused ion beam milling to make the microfluidic network and the in-plane pore sensor, respectively, in Si from which UV resin stamps were generated followed by thermal imprinting to produce the final device in the appropriate plastic (cyclic olefin polymer, COP). As an example of the utility of this in-plane extended nanopore sensor, we enumerated SARS-CoV-2 viral particles (VPs) affinity-selected from saliva and extracellular vesicles (EVs) affinity-selected from plasma samples secured from mouse models exposed to different ionizing radiation doses.

引用

页码：1961 / 1975

页数：15

共 4 条

[1] High Sensitivity Extended Nano-Coulter Counter for Detection of Viral Particles and Extracellular Vesicles
Vaidyanathan, Swarnagowri
Wijerathne, Harshani
Gamage, Sachindra S. T.
Shiri, Farhad
Zhao, Zheng
Choi, Junseo
Park, Sunggook
Witek, Malgorzata A.
McKinney, Collin
Verber, Matthew
Hall, Adam R.
Childers, Katie
McNickle, Taryn
Mog, Shalee
Yeh, Elaine
Godwin, Andrew K.
Soper, Steven A.
ANALYTICAL CHEMISTRY, 2023, 95 (26) : 9892 - 9900
[2] Label-free protein detection using a microfluidic Coulter-counter device
Rodriguez-Trujillo, Romen
Ajine, Mohammad A.
Orzan, Alice
Mar, Mikkel D.
Larsen, Flemming
Clausen, Casper H.
Svendsen, Winnie E.
SENSORS AND ACTUATORS B-CHEMICAL, 2014, 190 : 922 - 927
[3] Toward rapid detection of biological particles using multivariable resonant label-free biosensors
Potyrailo, Radislav A.
Sinton, David
Lee, Yongjae
Nosrati, Reza
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2013, 245
[4] Acute On-Chip HIV Detection Through Label-Free Electrical Sensing of Viral Nano-Lysate
Shafiee, Hadi
Jahangir, Muntasir
Inci, Fatih
Wang, ShuQi
Willenbrecht, Remington B. M.
Giguel, Francoise F.
Tsibris, Athe M. N.
Kuritzkes, Daniel R.
Demirci, Utkan
SMALL, 2013, 9 (15) : 2553 - 2563

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