Identification and discrimination of eight Greek grape cultivars (Vitis vinifera L.) by random amplified polymorphic DNA markers

被引：0
|
作者：
Stavrakakis, MN
Biniari, K
Hatzopoulos, P
机构：
[1] Agr Univ Athens, Lab Viticulture, GR-11855 Athens, Greece
[2] Agr Univ Athens, Mol Biol Lab, GR-11855 Athens, Greece
来源：
VITIS | 1997年 / 36卷 / 04期
关键词：
dendrogram; grape cultivars; PCR; RAPD markers; Vitis vinifera; similarity index;
D O I：
暂无
中图分类号：
S6 [园艺];
学科分类号：
0902 ;
摘要：
Fifteen decamer primers of an arbitrary nucleotide sequence were used to amplify genomic DNA by polymerase chain reaction (PCR-RAPD) in order to identify and discriminate between 8 cultivars of Vitis vinifera L., grown at the Island of Crete. Over 140 reproducible polymorphic fragments were generated by this method. Each grape cultivar showed a unique banding pattern for more than 5 of the primers used. Herefrom, the degree of genetic similarity was calculated and the dendrogram of the 8 cultivars was constructed. The results show that RAPD is a reliable and very useful method for the identification and genomic analysis of grape cultivars.
引用
收藏
页码：175 / 178
页数：4
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