Rhodohalobacter mucosus sp. nov., isolated from a marine solar saltern

A Gram-stain-negative, non-motile, aerobic, rod-shaped and reddish-pigmented bacterium, designated 8A47(T), was isolated from a marine solar saltern located in Wendeng, PR China. The novel strain 8A47(T) grows at 20-42 degrees C, pH 7.0-9.0, and in the presence of 2.0-14.0% (w/v) NaCl. Optimal growth was observed at 37-40 degrees C, pH 7.5-8.0, and with 4.0-6.0% (w/v) NaCl. The phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 8A47(T) formed an evolutionary lineage with members of the genus Rhodohalobacter. Strain 8A47(T) exhibited high level of similarity to Rhodohalobacter barlenses MCCC 1K03442(T) (94.7%) and Rhodohalobacter halophilus JZ3C29(T) (93.5%). The major fatty acids were summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c), C-16:0 and iso-C-15:0. The sole respiratory quinone was MK-7. The polar lipids of the new isolate consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified lipid, two unidentified phospholipids and three unidentified glycolipids and. The genomic DNA G + C content of the strain 8A47(T) was 47.7 mol%. Based on its phenotypic, chemotaxonomic, genotypic and genomic characteristics presented in this study, strain 8A47(T) is considered to represent a novel species of the genus Rhodohalobacter, for which the name Rhodohalobacter mucosus sp. nov. is proposed. The type strain is 8A47(T) (= KCTC 62603(T) = MCCC 1H00329(T)).