Design and implementation of web-based genotyping system of Newcastle disease viruse

According to the genotyping method of Newcastle disease virus (NDV), a web-based genotyping system is developed by using Java Server Pages (JSP) technology and MySQL server database technology. Users input the sequence file in FASTA or GenBank format, or pasted either the nucleotide or amino acid sequences of the F gene or the complete genome sequence of NDV into given textbox, then the system was performed to acquire the information of enzyme restriction sites and specific amino acid sites after analyzing the sequence automatically. The sites information was then compared with the genotyping standards and the genotype of NDV strains which belong to class II division was determined and displayed subsequently. In the validation test, representative sequences of genotypes I-IX of NDV strains were determined individually, and the results of genotyping were completely with the genotypes identified manually. The system is available online, without charge, at http://ndv.yzu.edu.cn/index.html.