ΔznuAΔpurE Brucella abortus 2308 mutant as a live vaccine candidate

To create a new, safe brucellosis live vaccine, a double Mutant strain was constructed from Brucella abortus 2308 Using the Delta znuA B abortus 2308 mutant, a second mutation was introduced by deleting purE gene The Delta znuA Delta purE B. abortus 2308 strain was less capable of surviving in macrophages When evaluated in vivo. it was cleared within 8 weeks (wks) from mice. causing significantly less inflammation than spleens obtained from wild-type B abortus 2308-infected mice Furthermore, two doses of Delta znuA Delta purE B abortus 2308 conferred 0 79 log protection, similar to S19 as did a single close of Delta znuA B abortus 2308 Thus, this study shows the Delta znuA Delta purE B abortus 2308 strain to be a potential livestock vaccine candidate. (C) 2009 Elsevier Ltd All rights reserved