High performance liquid chromatographic separation and UV determination of cobalt, copper iron and platinum in pharmaceutical preparations using Bis(isovalerylacetone)ethylenediimine as complexing reagent

The re agent bis(isovalerylacetone)ethylenediimine(H(2)IVA(2)en) has been examined for HPLC separation and UV determination of cobalt, copper, iron and platinum using off-line precolumn derivatization and extraction in chloroform. The complexes of cobalt(II), cobalt(III), iron(II), iron(III) and the reagent have been subsequently separated on a Microsorb C-18 column. The complexes were eluted isocratically using ternary mixtures of methanol/water/acetonitrile. Detection was achieved by UV monitoring. Detection limits for Co(II), Co(III), Fe(II) and Fe(III) were 2.5-5.0ng/injection, based on 0.5-1.0 mu g/ml with 5 mu l/injection. The concentration of cobalt(II) and cobalt(III) in aqueous solution have been determined. The presence of oxovanadium(IV), platinum(II), and nickel(II) did not affect the determinations. The HPLC method developed has been applied to the determination of cobalt, copper, iron and platinum in pharmaceutical preparations at the 30 mu g/g to 15 mg/g level and the obtained results were compared to those of atomic absorption spectrometry.