Measurements of sub-nanomolar concentrations of unmetabolised folic acid in serum

被引：27
|
作者：
Sweeney, MR
McPartlin, J
Weir, DG
Scott, JM
机构：
[1] Univ Dublin Trinity Coll, Dept Clin Med, Dublin 2, Ireland
[2] Univ Dublin Trinity Coll, Dept Biochem, Dublin 2, Ireland
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2003年 / 788卷 / 01期
关键词：
folic acid;
D O I：
10.1016/S1570-0232(02)01010-3
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We describe a combined HPLC/microbiological assay procedure for the sub-nanomolar analysis of unmetabolised folic acid (pteroylglutamate) in human serum. This metabolically unaltered form of the vitamin arises following the consumption of folic acid either in supplemental form or in fortified foods. Following HPLC separation of folic acid from other folate derivatives the folic acid fraction was concentrated by C-18 Sep-Pak cartridges and assayed by Lactobacillus casei microbiological assay. The present assay allows the quantitation and kinetic analysis of the effects of consumption of folic acid. (C) 2003 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：187 / 191
页数：5
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