The relationship between caveolin-1 isoforms (alpha and beta) and caveolar ultrastructure was studied, By immunofluorescence microscopy of human fibroblasts, caveolae were observed as dots positive for caveolin-1, but many dots labeled by an antibody recognizing both isoforms (anti-alpha beta) were not labeled hy another antibody specific for the alpha isoform (anti-alpha), Immunogold electron microscopy of freeze-fracture replicas revealed caveolae of different depths, and indicated that anti-ct labeled deep caveolae preferentially over shallow ones, whereas anti-ap labeled both forms with an equivalent frequency and intensity, The presence of the beta isoform in deep caveolae was confirmed by labeling elpitope-tagged beta -caveolin When made to be expressed in HepG2 cells lacking endogenous caveolins, the alpha isoform formed caveolar depressions efficiently, but the beta isoform hardly did so. Caveolae were also formed in cells expressing the two isoforms, but their frequency was variable among cells of the same clone. Coexpression of caveolin-1 and caveolin-2 caused more efficient formation of deep caveolae than caveolin-1 alone, The result indicates that the two isoforms of caveolin-1 have a different potential for forming caveolae structure, and more importantly, that deep and shallow caveolae may be diversified in their molecular composition.
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Univ Oklahoma, Hlth Sci Ctr, Physiol, Oklahoma City, OK USAUniv Oklahoma, Hlth Sci Ctr, Physiol, Oklahoma City, OK USA
Bankole, Olawale
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Gurley, Jami
Ash, John D.
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Univ Pittsburgh, Sch Med, Ophthalmol, Pittsburgh, PA USAUniv Oklahoma, Hlth Sci Ctr, Physiol, Oklahoma City, OK USA
Ash, John D.
Elliott, Michael H.
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Univ Oklahoma, Hlth Sci Ctr, Physiol, Oklahoma City, OK USA
Dean McGee Eye Inst, Ophthalmol, Oklahoma City, OK USAUniv Oklahoma, Hlth Sci Ctr, Physiol, Oklahoma City, OK USA
机构:Chinese Center for Disease Control and Prevention,State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention
Qi Shi
Yuan-Yuan Jing
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Yuan-Yuan Jing
Shao-Bin Wang
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机构:Chinese Center for Disease Control and Prevention,State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention
Shao-Bin Wang
Cao Chen
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机构:Chinese Center for Disease Control and Prevention,State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention
Cao Chen
Han Sun
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机构:Chinese Center for Disease Control and Prevention,State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention
Han Sun
Yin Xu
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机构:Chinese Center for Disease Control and Prevention,State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention
Yin Xu
Chen Gao
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机构:Chinese Center for Disease Control and Prevention,State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention
Chen Gao
Jin Zhang
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机构:Chinese Center for Disease Control and Prevention,State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention
Jin Zhang
Chan Tian
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机构:Chinese Center for Disease Control and Prevention,State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention
Chan Tian
Yan Guo
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机构:Chinese Center for Disease Control and Prevention,State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention
Yan Guo
Ke Ren
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机构:Chinese Center for Disease Control and Prevention,State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention
Ke Ren
Xiao-Ping Dong
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机构:Chinese Center for Disease Control and Prevention,State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention
Xiao-Ping Dong
Medical Microbiology and Immunology,
2013,
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